cd44 (Cell Signaling Technology Inc)
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cd44
Cd44, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 790 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-cd44/CD44+Antibody/pmc13052523-51-17-26
Average 96 stars, based on 790 article reviews
Cd44, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 790 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-cd44/CD44+Antibody/pmc13052523-51-17-26
Average 96 stars, based on 790 article reviews
cd44 - by Bioz Stars,
2026-10
96/100 stars
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Membrane:Article Title: Cell flocculation and phase-separation support macro-scale tissue slab construction in a scaffold-free manner Article Snippet: The membrane was blocked in 5 % skim milk for 1 h at room temperature and incubated with 2 mL of primary Article Title: Raphanus sativus L. seed extracts induce apoptosis and reduce migration of oral squamous cell carcinoma KB and KB CD133+ cells by downregulation of β-catenin. Article Snippet: Although, oral cancer therapies have been developed for decades, patient survival rates have not changed.. Side effects of chemotherapy and radiotherapy reduce quality of life of patients and it remains difficult to treat oral cancers due to the presence of cancer stem cells (CSCs) that cause recurrence and metastasis.. Therefore, we search for natural products that affect oral cancer cells including oral cancer stem cells. Article Title: MiR-1976 knockdown promotes epithelial-mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis. Article Snippet: The primary antibodies included anti-E-cadherin, anti-Ncadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination Article Snippet: Proteins of interest were identified with an anti-CD44 (1:2000; Cat# 3570, Cell Signaling), anti-acetyl-histone H4 (Lys5) (1:20000; Cat# 07-327, Millipore), anti-acetyl-histone H4 (Lys12) (1:5000; Cat# 07-595, Millipore), anti-acetyl-histone H4 (Lys16) (1:3000; Cat# 07-329, Millipore), anti-Actin (1:10000; Cat# MAB1501, Millipore), anti-ATF4 (1:500; Cat# 11815, Cell Signaling), anti-Chk1 (1:2000; Cat# 2345, Cell Signaling), anti-cyclin A (1:1000; Cat# sc-751, Santa Cruz), anti-cyclin B1 (1:1000; Cat# ADI-Kam-CC195, Enzo), anti-eIF2α (1:2000; Cat# 9722, Cell Signaling), anti-γH2AX (1:3000; Cat# A300-081, Bethyl), anti-histone H3 (1:20000; Cat# 39763, Active Motif), anti-histone H4 (1:10000 or 1:500 for long exposure; Cat# ab177840, abcam), anti-lamin A/C (1:2000, Cat# 4777, Cell Signaling), anti-BRCA2 (1:3000, Cat# ab27976, abcam), anti-PCNA (1:3000, Cat# sc-7907, Santa Cruz), anti-phospho-eIF2α (1:1000; Cat# 9721S, Cell Signaling), anti-phospho-Chk1 (Ser296) (1:2000, Cat# ab79758, abcam), anti-phospho-Chk1 (Ser345) (1:2000; Cat# 2348, Cell Signaling), anti-phospho-histone H3 (Ser10) (1:1000; Cat# 06-570, Millipore Sigma), anti-RPA32/RPA2 (1:1000, Cat# ab2175, abcam), anti-phospho-RPA32 (Ser33) (1:3000; Cat# A300-246A, Bethyl Laboratories), anti-trimethyl-histone H3 (Lys4) (1:2000; Cat# Article Title: MiR-1976 knockdown promotes epithelial–mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis Article Snippet: The primary antibodies included anti-E-cadherin, anti-N-cadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Glioma-initiating cells at tumor edge gain signals from tumor core cells to promote their malignancy Article Snippet: The following antibodies were used in the study (Epitope, Supplier, catalogue number, experiment type, host) anti-CD44 (Cell Signaling, 3570; IHC, WB, IF; mouse), anti-GAPDH (Abcam, ab9485 ; WB; rabbit), Article Title: Methods of using thrombin derivatives to treat medulloblastoma Article Snippet: Alexa Fluor-594 and Alexa Fluor-488 coupled secondary IgG were from Invitrogen (Carlsbad, Calif.). Article Title: Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines. Article Snippet: Accepted Manuscript Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson PII: S1570-9639(18)30206-1 DOI: https://doi.org/10.1016/j.bbapap.2018.11.008 Reference: BBAPAP 40171 To appear in: BBA - Proteins and Proteomics Received date: 3 August 2018 Revised date: 5 November 2018 Accepted date: 23 November 2018 Please cite this article as: Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson , Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines.. Bbapap (2018), https://doi.org/10.1016/j.bbapap.2018.11.008 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript. Incubation:Article Title: Cell flocculation and phase-separation support macro-scale tissue slab construction in a scaffold-free manner Article Snippet: The membrane was blocked in 5 % skim milk for 1 h at room temperature and incubated with 2 mL of primary Article Title: Raphanus sativus L. seed extracts induce apoptosis and reduce migration of oral squamous cell carcinoma KB and KB CD133+ cells by downregulation of β-catenin. Article Snippet: Although, oral cancer therapies have been developed for decades, patient survival rates have not changed.. Side effects of chemotherapy and radiotherapy reduce quality of life of patients and it remains difficult to treat oral cancers due to the presence of cancer stem cells (CSCs) that cause recurrence and metastasis.. Therefore, we search for natural products that affect oral cancer cells including oral cancer stem cells. Article Title: MiR-1976 knockdown promotes epithelial-mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis. Article Snippet: The primary antibodies included anti-E-cadherin, anti-Ncadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination Article Snippet: Proteins of interest were identified with an anti-CD44 (1:2000; Cat# 3570, Cell Signaling), anti-acetyl-histone H4 (Lys5) (1:20000; Cat# 07-327, Millipore), anti-acetyl-histone H4 (Lys12) (1:5000; Cat# 07-595, Millipore), anti-acetyl-histone H4 (Lys16) (1:3000; Cat# 07-329, Millipore), anti-Actin (1:10000; Cat# MAB1501, Millipore), anti-ATF4 (1:500; Cat# 11815, Cell Signaling), anti-Chk1 (1:2000; Cat# 2345, Cell Signaling), anti-cyclin A (1:1000; Cat# sc-751, Santa Cruz), anti-cyclin B1 (1:1000; Cat# ADI-Kam-CC195, Enzo), anti-eIF2α (1:2000; Cat# 9722, Cell Signaling), anti-γH2AX (1:3000; Cat# A300-081, Bethyl), anti-histone H3 (1:20000; Cat# 39763, Active Motif), anti-histone H4 (1:10000 or 1:500 for long exposure; Cat# ab177840, abcam), anti-lamin A/C (1:2000, Cat# 4777, Cell Signaling), anti-BRCA2 (1:3000, Cat# ab27976, abcam), anti-PCNA (1:3000, Cat# sc-7907, Santa Cruz), anti-phospho-eIF2α (1:1000; Cat# 9721S, Cell Signaling), anti-phospho-Chk1 (Ser296) (1:2000, Cat# ab79758, abcam), anti-phospho-Chk1 (Ser345) (1:2000; Cat# 2348, Cell Signaling), anti-phospho-histone H3 (Ser10) (1:1000; Cat# 06-570, Millipore Sigma), anti-RPA32/RPA2 (1:1000, Cat# ab2175, abcam), anti-phospho-RPA32 (Ser33) (1:3000; Cat# A300-246A, Bethyl Laboratories), anti-trimethyl-histone H3 (Lys4) (1:2000; Cat# Article Title: MiR-1976 knockdown promotes epithelial–mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis Article Snippet: The primary antibodies included anti-E-cadherin, anti-N-cadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Glioma-initiating cells at tumor edge gain signals from tumor core cells to promote their malignancy Article Snippet: The following antibodies were used in the study (Epitope, Supplier, catalogue number, experiment type, host) anti-CD44 (Cell Signaling, 3570; IHC, WB, IF; mouse), anti-GAPDH (Abcam, ab9485 ; WB; rabbit), Article Title: Methods of using thrombin derivatives to treat medulloblastoma Article Snippet: Alexa Fluor-594 and Alexa Fluor-488 coupled secondary IgG were from Invitrogen (Carlsbad, Calif.). Article Title: Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines. Article Snippet: Accepted Manuscript Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson PII: S1570-9639(18)30206-1 DOI: https://doi.org/10.1016/j.bbapap.2018.11.008 Reference: BBAPAP 40171 To appear in: BBA - Proteins and Proteomics Received date: 3 August 2018 Revised date: 5 November 2018 Accepted date: 23 November 2018 Please cite this article as: Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson , Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines.. Bbapap (2018), https://doi.org/10.1016/j.bbapap.2018.11.008 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript. Immunohistochemistry:Article Title: Cell flocculation and phase-separation support macro-scale tissue slab construction in a scaffold-free manner Article Snippet: The membrane was blocked in 5 % skim milk for 1 h at room temperature and incubated with 2 mL of primary Article Title: Raphanus sativus L. seed extracts induce apoptosis and reduce migration of oral squamous cell carcinoma KB and KB CD133+ cells by downregulation of β-catenin. Article Snippet: Although, oral cancer therapies have been developed for decades, patient survival rates have not changed.. Side effects of chemotherapy and radiotherapy reduce quality of life of patients and it remains difficult to treat oral cancers due to the presence of cancer stem cells (CSCs) that cause recurrence and metastasis.. Therefore, we search for natural products that affect oral cancer cells including oral cancer stem cells. Article Title: MiR-1976 knockdown promotes epithelial-mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis. Article Snippet: The primary antibodies included anti-E-cadherin, anti-Ncadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination Article Snippet: Proteins of interest were identified with an anti-CD44 (1:2000; Cat# 3570, Cell Signaling), anti-acetyl-histone H4 (Lys5) (1:20000; Cat# 07-327, Millipore), anti-acetyl-histone H4 (Lys12) (1:5000; Cat# 07-595, Millipore), anti-acetyl-histone H4 (Lys16) (1:3000; Cat# 07-329, Millipore), anti-Actin (1:10000; Cat# MAB1501, Millipore), anti-ATF4 (1:500; Cat# 11815, Cell Signaling), anti-Chk1 (1:2000; Cat# 2345, Cell Signaling), anti-cyclin A (1:1000; Cat# sc-751, Santa Cruz), anti-cyclin B1 (1:1000; Cat# ADI-Kam-CC195, Enzo), anti-eIF2α (1:2000; Cat# 9722, Cell Signaling), anti-γH2AX (1:3000; Cat# A300-081, Bethyl), anti-histone H3 (1:20000; Cat# 39763, Active Motif), anti-histone H4 (1:10000 or 1:500 for long exposure; Cat# ab177840, abcam), anti-lamin A/C (1:2000, Cat# 4777, Cell Signaling), anti-BRCA2 (1:3000, Cat# ab27976, abcam), anti-PCNA (1:3000, Cat# sc-7907, Santa Cruz), anti-phospho-eIF2α (1:1000; Cat# 9721S, Cell Signaling), anti-phospho-Chk1 (Ser296) (1:2000, Cat# ab79758, abcam), anti-phospho-Chk1 (Ser345) (1:2000; Cat# 2348, Cell Signaling), anti-phospho-histone H3 (Ser10) (1:1000; Cat# 06-570, Millipore Sigma), anti-RPA32/RPA2 (1:1000, Cat# ab2175, abcam), anti-phospho-RPA32 (Ser33) (1:3000; Cat# A300-246A, Bethyl Laboratories), anti-trimethyl-histone H3 (Lys4) (1:2000; Cat# Article Title: MiR-1976 knockdown promotes epithelial–mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis Article Snippet: The primary antibodies included anti-E-cadherin, anti-N-cadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Glioma-initiating cells at tumor edge gain signals from tumor core cells to promote their malignancy Article Snippet: The following antibodies were used in the study (Epitope, Supplier, catalogue number, experiment type, host) anti-CD44 (Cell Signaling, 3570; IHC, WB, IF; mouse), anti-GAPDH (Abcam, ab9485 ; WB; rabbit), Article Title: Methods of using thrombin derivatives to treat medulloblastoma Article Snippet: Alexa Fluor-594 and Alexa Fluor-488 coupled secondary IgG were from Invitrogen (Carlsbad, Calif.). Article Title: Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines. Article Snippet: Accepted Manuscript Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson PII: S1570-9639(18)30206-1 DOI: https://doi.org/10.1016/j.bbapap.2018.11.008 Reference: BBAPAP 40171 To appear in: BBA - Proteins and Proteomics Received date: 3 August 2018 Revised date: 5 November 2018 Accepted date: 23 November 2018 Please cite this article as: Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson , Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines.. Bbapap (2018), https://doi.org/10.1016/j.bbapap.2018.11.008 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript. Western Blot:Article Title: Cell flocculation and phase-separation support macro-scale tissue slab construction in a scaffold-free manner Article Snippet: The membrane was blocked in 5 % skim milk for 1 h at room temperature and incubated with 2 mL of primary Article Title: Raphanus sativus L. seed extracts induce apoptosis and reduce migration of oral squamous cell carcinoma KB and KB CD133+ cells by downregulation of β-catenin. Article Snippet: Although, oral cancer therapies have been developed for decades, patient survival rates have not changed.. Side effects of chemotherapy and radiotherapy reduce quality of life of patients and it remains difficult to treat oral cancers due to the presence of cancer stem cells (CSCs) that cause recurrence and metastasis.. Therefore, we search for natural products that affect oral cancer cells including oral cancer stem cells. Article Title: MiR-1976 knockdown promotes epithelial-mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis. Article Snippet: The primary antibodies included anti-E-cadherin, anti-Ncadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination Article Snippet: Proteins of interest were identified with an anti-CD44 (1:2000; Cat# 3570, Cell Signaling), anti-acetyl-histone H4 (Lys5) (1:20000; Cat# 07-327, Millipore), anti-acetyl-histone H4 (Lys12) (1:5000; Cat# 07-595, Millipore), anti-acetyl-histone H4 (Lys16) (1:3000; Cat# 07-329, Millipore), anti-Actin (1:10000; Cat# MAB1501, Millipore), anti-ATF4 (1:500; Cat# 11815, Cell Signaling), anti-Chk1 (1:2000; Cat# 2345, Cell Signaling), anti-cyclin A (1:1000; Cat# sc-751, Santa Cruz), anti-cyclin B1 (1:1000; Cat# ADI-Kam-CC195, Enzo), anti-eIF2α (1:2000; Cat# 9722, Cell Signaling), anti-γH2AX (1:3000; Cat# A300-081, Bethyl), anti-histone H3 (1:20000; Cat# 39763, Active Motif), anti-histone H4 (1:10000 or 1:500 for long exposure; Cat# ab177840, abcam), anti-lamin A/C (1:2000, Cat# 4777, Cell Signaling), anti-BRCA2 (1:3000, Cat# ab27976, abcam), anti-PCNA (1:3000, Cat# sc-7907, Santa Cruz), anti-phospho-eIF2α (1:1000; Cat# 9721S, Cell Signaling), anti-phospho-Chk1 (Ser296) (1:2000, Cat# ab79758, abcam), anti-phospho-Chk1 (Ser345) (1:2000; Cat# 2348, Cell Signaling), anti-phospho-histone H3 (Ser10) (1:1000; Cat# 06-570, Millipore Sigma), anti-RPA32/RPA2 (1:1000, Cat# ab2175, abcam), anti-phospho-RPA32 (Ser33) (1:3000; Cat# A300-246A, Bethyl Laboratories), anti-trimethyl-histone H3 (Lys4) (1:2000; Cat# Article Title: MiR-1976 knockdown promotes epithelial–mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis Article Snippet: The primary antibodies included anti-E-cadherin, anti-N-cadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Glioma-initiating cells at tumor edge gain signals from tumor core cells to promote their malignancy Article Snippet: The following antibodies were used in the study (Epitope, Supplier, catalogue number, experiment type, host) anti-CD44 (Cell Signaling, 3570; IHC, WB, IF; mouse), anti-GAPDH (Abcam, ab9485 ; WB; rabbit), Article Title: Methods of using thrombin derivatives to treat medulloblastoma Article Snippet: Alexa Fluor-594 and Alexa Fluor-488 coupled secondary IgG were from Invitrogen (Carlsbad, Calif.). Article Title: Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines. Article Snippet: Accepted Manuscript Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson PII: S1570-9639(18)30206-1 DOI: https://doi.org/10.1016/j.bbapap.2018.11.008 Reference: BBAPAP 40171 To appear in: BBA - Proteins and Proteomics Received date: 3 August 2018 Revised date: 5 November 2018 Accepted date: 23 November 2018 Please cite this article as: Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson , Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines.. Bbapap (2018), https://doi.org/10.1016/j.bbapap.2018.11.008 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript. Chromatin Immunoprecipitation:Article Title: Cell flocculation and phase-separation support macro-scale tissue slab construction in a scaffold-free manner Article Snippet: The membrane was blocked in 5 % skim milk for 1 h at room temperature and incubated with 2 mL of primary Article Title: Raphanus sativus L. seed extracts induce apoptosis and reduce migration of oral squamous cell carcinoma KB and KB CD133+ cells by downregulation of β-catenin. Article Snippet: Although, oral cancer therapies have been developed for decades, patient survival rates have not changed.. Side effects of chemotherapy and radiotherapy reduce quality of life of patients and it remains difficult to treat oral cancers due to the presence of cancer stem cells (CSCs) that cause recurrence and metastasis.. Therefore, we search for natural products that affect oral cancer cells including oral cancer stem cells. Article Title: MiR-1976 knockdown promotes epithelial-mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis. Article Snippet: The primary antibodies included anti-E-cadherin, anti-Ncadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination Article Snippet: Proteins of interest were identified with an anti-CD44 (1:2000; Cat# 3570, Cell Signaling), anti-acetyl-histone H4 (Lys5) (1:20000; Cat# 07-327, Millipore), anti-acetyl-histone H4 (Lys12) (1:5000; Cat# 07-595, Millipore), anti-acetyl-histone H4 (Lys16) (1:3000; Cat# 07-329, Millipore), anti-Actin (1:10000; Cat# MAB1501, Millipore), anti-ATF4 (1:500; Cat# 11815, Cell Signaling), anti-Chk1 (1:2000; Cat# 2345, Cell Signaling), anti-cyclin A (1:1000; Cat# sc-751, Santa Cruz), anti-cyclin B1 (1:1000; Cat# ADI-Kam-CC195, Enzo), anti-eIF2α (1:2000; Cat# 9722, Cell Signaling), anti-γH2AX (1:3000; Cat# A300-081, Bethyl), anti-histone H3 (1:20000; Cat# 39763, Active Motif), anti-histone H4 (1:10000 or 1:500 for long exposure; Cat# ab177840, abcam), anti-lamin A/C (1:2000, Cat# 4777, Cell Signaling), anti-BRCA2 (1:3000, Cat# ab27976, abcam), anti-PCNA (1:3000, Cat# sc-7907, Santa Cruz), anti-phospho-eIF2α (1:1000; Cat# 9721S, Cell Signaling), anti-phospho-Chk1 (Ser296) (1:2000, Cat# ab79758, abcam), anti-phospho-Chk1 (Ser345) (1:2000; Cat# 2348, Cell Signaling), anti-phospho-histone H3 (Ser10) (1:1000; Cat# 06-570, Millipore Sigma), anti-RPA32/RPA2 (1:1000, Cat# ab2175, abcam), anti-phospho-RPA32 (Ser33) (1:3000; Cat# A300-246A, Bethyl Laboratories), anti-trimethyl-histone H3 (Lys4) (1:2000; Cat# Article Title: MiR-1976 knockdown promotes epithelial–mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis Article Snippet: The primary antibodies included anti-E-cadherin, anti-N-cadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Glioma-initiating cells at tumor edge gain signals from tumor core cells to promote their malignancy Article Snippet: The following antibodies were used in the study (Epitope, Supplier, catalogue number, experiment type, host) anti-CD44 (Cell Signaling, 3570; IHC, WB, IF; mouse), anti-GAPDH (Abcam, ab9485 ; WB; rabbit), Article Title: Methods of using thrombin derivatives to treat medulloblastoma Article Snippet: Alexa Fluor-594 and Alexa Fluor-488 coupled secondary IgG were from Invitrogen (Carlsbad, Calif.). Article Title: Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines. Article Snippet: Accepted Manuscript Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson PII: S1570-9639(18)30206-1 DOI: https://doi.org/10.1016/j.bbapap.2018.11.008 Reference: BBAPAP 40171 To appear in: BBA - Proteins and Proteomics Received date: 3 August 2018 Revised date: 5 November 2018 Accepted date: 23 November 2018 Please cite this article as: Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson , Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines.. Bbapap (2018), https://doi.org/10.1016/j.bbapap.2018.11.008 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript. Biomarker Discovery:Article Title: Cell flocculation and phase-separation support macro-scale tissue slab construction in a scaffold-free manner Article Snippet: The membrane was blocked in 5 % skim milk for 1 h at room temperature and incubated with 2 mL of primary Article Title: Raphanus sativus L. seed extracts induce apoptosis and reduce migration of oral squamous cell carcinoma KB and KB CD133+ cells by downregulation of β-catenin. Article Snippet: Although, oral cancer therapies have been developed for decades, patient survival rates have not changed.. Side effects of chemotherapy and radiotherapy reduce quality of life of patients and it remains difficult to treat oral cancers due to the presence of cancer stem cells (CSCs) that cause recurrence and metastasis.. Therefore, we search for natural products that affect oral cancer cells including oral cancer stem cells. Article Title: MiR-1976 knockdown promotes epithelial-mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis. Article Snippet: The primary antibodies included anti-E-cadherin, anti-Ncadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination Article Snippet: Proteins of interest were identified with an anti-CD44 (1:2000; Cat# 3570, Cell Signaling), anti-acetyl-histone H4 (Lys5) (1:20000; Cat# 07-327, Millipore), anti-acetyl-histone H4 (Lys12) (1:5000; Cat# 07-595, Millipore), anti-acetyl-histone H4 (Lys16) (1:3000; Cat# 07-329, Millipore), anti-Actin (1:10000; Cat# MAB1501, Millipore), anti-ATF4 (1:500; Cat# 11815, Cell Signaling), anti-Chk1 (1:2000; Cat# 2345, Cell Signaling), anti-cyclin A (1:1000; Cat# sc-751, Santa Cruz), anti-cyclin B1 (1:1000; Cat# ADI-Kam-CC195, Enzo), anti-eIF2α (1:2000; Cat# 9722, Cell Signaling), anti-γH2AX (1:3000; Cat# A300-081, Bethyl), anti-histone H3 (1:20000; Cat# 39763, Active Motif), anti-histone H4 (1:10000 or 1:500 for long exposure; Cat# ab177840, abcam), anti-lamin A/C (1:2000, Cat# 4777, Cell Signaling), anti-BRCA2 (1:3000, Cat# ab27976, abcam), anti-PCNA (1:3000, Cat# sc-7907, Santa Cruz), anti-phospho-eIF2α (1:1000; Cat# 9721S, Cell Signaling), anti-phospho-Chk1 (Ser296) (1:2000, Cat# ab79758, abcam), anti-phospho-Chk1 (Ser345) (1:2000; Cat# 2348, Cell Signaling), anti-phospho-histone H3 (Ser10) (1:1000; Cat# 06-570, Millipore Sigma), anti-RPA32/RPA2 (1:1000, Cat# ab2175, abcam), anti-phospho-RPA32 (Ser33) (1:3000; Cat# A300-246A, Bethyl Laboratories), anti-trimethyl-histone H3 (Lys4) (1:2000; Cat# Article Title: MiR-1976 knockdown promotes epithelial–mesenchymal transition and cancer stem cell properties inducing triple-negative breast cancer metastasis Article Snippet: The primary antibodies included anti-E-cadherin, anti-N-cadherin, anti-Vimentin, anti-Snail, anti-Slug, anti-CD44, anti-PIK3CG, anti-AKT, anti-p-AKT and Article Title: Glioma-initiating cells at tumor edge gain signals from tumor core cells to promote their malignancy Article Snippet: The following antibodies were used in the study (Epitope, Supplier, catalogue number, experiment type, host) anti-CD44 (Cell Signaling, 3570; IHC, WB, IF; mouse), anti-GAPDH (Abcam, ab9485 ; WB; rabbit), Article Title: Methods of using thrombin derivatives to treat medulloblastoma Article Snippet: Alexa Fluor-594 and Alexa Fluor-488 coupled secondary IgG were from Invitrogen (Carlsbad, Calif.). Article Title: Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines. Article Snippet: Accepted Manuscript Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson PII: S1570-9639(18)30206-1 DOI: https://doi.org/10.1016/j.bbapap.2018.11.008 Reference: BBAPAP 40171 To appear in: BBA - Proteins and Proteomics Received date: 3 August 2018 Revised date: 5 November 2018 Accepted date: 23 November 2018 Please cite this article as: Wittaya Suwakulsiri, Alin Rai, Rong Xu, Maoshan Chen, David W. Greening, Richard J. Simpson , Proteomic profiling reveals key cancer progression modulators in shed microvesicles released from isogenic human primary and metastatic colorectal cancer cell lines.. Bbapap (2018), https://doi.org/10.1016/j.bbapap.2018.11.008 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript. |
